The tobacco plant has been made resistant to the tobacco mosaic virus by:
1. introducing T -DNA of Agrobacterium
2. introducing a gene for vims coat protein
3. introducing meristematic activity in somatic cells
4. applying newer generation pesticides to the crops
Plants are more easily manipulated by genetic engineering than are animals because:
1. Plant genes do not contain introns
2. More vectors are available to transfer rDNA into plant cells
3. Totipotency is a feature generally associated with plant cells and not with animal cells
4. Genes can be inserted into animal cells by microinjection.
Identify the incorrectly matched pair :
1. |
Humulin |
First therapeutic rDNA product approved for human use |
2. |
RNAi |
Silencing of mRNA with the help of dsRNA |
3. |
Rosie |
Transgenic sheep producing alpha 1 antitrypsin |
4. |
Golden rice |
Vitamin A enriched rice variety |
RNAi is a mechanism to silence genes with the help of
1. ssRNA
2. dsRNA
3. ssDNA
4. dsDNA
To protect tobacco plants against Meloidegyne incognita using RNAi, nematode specific genes were introduced in the plant using:
1. Transposons
2. Retrotransposons
3. Virus with RNA genome
4. Agrobacterium
A permanent cure for ADA deficiency could be:
1. | Enzyme replacement therapy |
2. | Bone marrow transplant |
3. | Introduction of a functional ADA cDNA into lymphocytes |
4. | Introduction of gene isolated from marrow cells producing ADA into early embryonic cells |
Polymerase Chain Reaction is not used in :
1. Confirming presence of a pathogen during early infection
2. Identifying the mutated genes in suspected cancer patients
3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures
4. Detection of the presence of HIV in suspected AIDS patient
If gene isolate from marrow cells producing ADA is introduced into cells at early embryonic stage, it could be a permanant cure of ADA deficiency because the early embryonic cells :
1. Are more differentiated than the later stage cells
2. Are constantly dividing and do not get differentiated
3. Are easy to manipulate than the later stage cells
4. Are virtually immortal
To test the safety of polio vaccine, the organisms used are transgenic :
1. Mice
2. Pigs
3. Monkey
4. Cattle
If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective ?
(1) clonal propagation
(2) cybridization
(3) protoplast fusion
(4) mutant selection