Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. |
Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. |
List I | List II | ||
A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm |
B. | Cry IAb | II. | ADA deficiency |
C. | Cry IAc | III. | Emphysema |
D. | Enzyme replacement therapy | IV. | Corn borer |
Statement I: | Two DNA sequences were prepared corresponding to A and B, chains of human insulin and were introduced in the plasmids of Agrobacterium to produce insulin chain. |
Statement II: | Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. |
Assertion (A): | Some ethical standards are required to evaluate the morality of scientific human activities that might help or harm living organisms. |
Reason (R): | Genetic Engineering Approval Committee make decisions regarding the validity of Genetically Modified (GM) research and the safety of introducing GM organisms for public services. |
1. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
3. | (A) is correct but (R) is not correct |
4. | (A) is not correct but (R) is correct. |
1. | Bt toxin requires acidic pH for activation |
2. | Bacillus has protective mechanism against it |
3. | In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form |
4. | The protoxin requires protein conjugate for its activation |
Statement I: | The Indian Government has set up GEAC, which will make decisions regarding the validity of GM research. |
Statement II: | Biopiracy is the term used to refer to the use of bio-resources by native people |
A. | The inactive toxin converted into active form due to alkaline pH of gut of insect |
B. | Bacillus thuringiensis produce crystals with toxic insecticidal proteins. |
C. | The alkaline pH solubilises the crystals. |
D. | The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. |
E. | The toxin proteins exist as inactive protoxins in bacteria. |