Q. No.Production of human protein in bacterial genetic engineering is possible because
1. Human chromosome replicate bacterial cell
2. Mechanism of gene regulation is identical in humans and bacteria
3. Bacterial cell can undertake RNA splicing
4. Genetic code is universal
1. Human chromosome replicate bacterial cell
2. Mechanism of gene regulation is identical in humans and bacteria
3. Bacterial cell can undertake RNA splicing
4. Genetic code is universal
The linking of antibiotic resistance gene with the plasmid vector became possible due to
1. DNA polymerase
2. Exonucleases
3. DNA ligase
4. Endonucleases
Given below is a sample of a portion of DNA strand. What is so special shown in it
5' GAATTC 3'
3' CTTAAG 5'
1. Replication completed
2. Deletion mutation
3. Start codon at the 5' end
4. Palindromic sequence of base pairs
There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
1. Colon
2. Coelom
3. Coenzyme
4. coli
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Agarose extracted from sea weeds finds use in
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
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Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
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For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
| 1. | Silver or Platinum | 2. | Platinum or Zinc |
| 3. | Silicon or Platinum | 4. | Gold or Tungsten |
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Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in the recipient cell
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature
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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?
1. ori - original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, EcoRI - selectable markers
4. ampR, tetR- antibiotic resistance genes
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The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
Options:
1. B - Denaturation at a temperature of about 98°C separating the two DNA strands.
2. A - Denaturation at a temperature of about 50°C
3. C - Extension in the presence of heat-stable DNA polymerase
4. A - Annealing with two sets of primers
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