Q. No.Agarose extracted from sea weeds finds use in
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
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Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
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For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
| 1. | Silver or Platinum | 2. | Platinum or Zinc |
| 3. | Silicon or Platinum | 4. | Gold or Tungsten |
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Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in the recipient cell
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature
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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?
1. ori - original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, EcoRI - selectable markers
4. ampR, tetR- antibiotic resistance genes
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The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
Options:
1. B - Denaturation at a temperature of about 98°C separating the two DNA strands.
2. A - Denaturation at a temperature of about 50°C
3. C - Extension in the presence of heat-stable DNA polymerase
4. A - Annealing with two sets of primers
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Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'
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Biolistics (gene-gun) is suitable for:
| 1. | Disarming pathogen vectors |
| 2. | Transformation of plant cells |
| 3. | Constructing recombinant DNA by joining with vectors |
| 4. | DNA fingerprinting |
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In genetic engineering, the antibiotic resistance gene are used:
1. As selectable markers
2. To select healthy vectors
3. As sequences from where replication starts
4. to keep the cultures free of infection
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
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