Q. No.In genetic engineering, the antibiotic resistance gene are used:
1. As selectable markers
2. To select healthy vectors
3. As sequences from where replication starts
4. to keep the cultures free of infection
1. As selectable markers
2. To select healthy vectors
3. As sequences from where replication starts
4. to keep the cultures free of infection
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII |
| 3. | SmaI | 4. | BamHI |
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Which of the following cannot be used as a vector in rDNA technology?
| 1. | Plasmid | 2. | Phage DNA |
| 3. | Bacterium | 4. | YAC |
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The following palindrome is recognized by the restriction enzyme:
| 1. | BamHI | 2. | EcoRI |
| 3. | HindII | 4. | PstI |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
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A method used only for transforming animal cells is:
| 1. | Biolistics | 2. | Microinjection |
| 3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
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