Q. No.Which of the following cannot be used as a vector in rDNA technology?
1. Plasmid 2. Phage DNA 3. Bacterium 4. YAC
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The following palindrome is recognized by the restriction enzyme:
| 1. | BamHI | 2. | EcoRI |
| 3. | HindII | 4. | PstI |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
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A method used only for transforming animal cells is:
| 1. | Biolistics | 2. | Microinjection |
| 3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
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In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?
| 1. | Lipase | 2. | Protease |
| 3. | Exonuclease | 4. | Endonuclease |
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Restriction endonuclease
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
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Two microbes found to be very useful in gene engineering are
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans.
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