Q. No.Lysozyme should be used when isolating DNA in a pure form from:
1.
A bacterial cell
2.
A fungal cell
3.
A plant cell
4.
An animal cell
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In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?
| 1. | Lipase | 2. | Protease |
| 3. | Exonuclease | 4. | Endonuclease |
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Restriction endonuclease
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
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Two microbes found to be very useful in gene engineering are
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans.
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Gel electrophoresis is used for
1. Isolation of DNA molecules.
2. Cutting of DNA into fragments.
3. Separation of DNA fragments according to their size.
4. Construction of recombinant DNA by joining with cloning vector.
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Which one of the following is commonly used in the transfer of foreign DNA into crop plants?
1. Penicilliumexpansum
2. Trichodermaharzianum
3. Meloidogyne incognita
4. Agrobacterium tumefaciens
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Polyethylene glycol method is used for
| 1. | Energy production from sewage. |
| 2. | Gene transfer without a vector |
| 3. | Biodiesel production. |
| 4. | Seedless fruit production. |
Which one of the following is used as a vector for cloning genes into higher organisms?
1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopusnigricans
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Restriction endonucleases are enzymes which
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
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