Q. No.Restriction endonucleases are enzymes which
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1. 5’______CACGTA_______3’
3’______CTVAGT_______5’
2. 5’______CGTTCG_______3’
3’______ATGGTA_______5’
3. 5’______GATATG_______3’
3’______CTACTA_______5’
4. 5’______GAATTC_______3’
3’______CTTAAG_______5’
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Stirred-tank bioreactors have been designed for:
1. Availability of oxygen throughout the process
2. Addition of preservatives to the product
3. Purification of the product
4. Ensuring anaerobic conditions in the culture vessel
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Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
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Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosomes
2. Yeast artificial chromosomes
3. Plasmid
4. Cosmid
The cutting of DNA at specific locations became possible with the discovery of
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
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Which of the following is not a feature of the plasmids?
1. Independent replication
2. Circular structure
3. Transferable
4. Single-stranded
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The Taq polymerase enzyme is obtained from:
1. Thermus aquaticus
2. Thiobacillus ferrooxidans
3. Bacillus subtilis
4. Pseudomonas putida
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Which of the following is not a component of downstream processing?
1. Purification
2. Preservation
3. Expression
4. Separation
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
| 1. | Taq polymerase | 2. | Polymerase III |
| 3. | Ligase | 4. | EcoRI |
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