Q. No.The Taq polymerase enzyme is obtained from:
1. Thermus aquaticus
2. Thiobacillus ferrooxidans
3. Bacillus subtilis
4. Pseudomonas putida
1. Thermus aquaticus
2. Thiobacillus ferrooxidans
3. Bacillus subtilis
4. Pseudomonas putida
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Which of the following is not a component of downstream processing?
1. Purification
2. Preservation
3. Expression
4. Separation
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
| 1. | Taq polymerase | 2. | Polymerase III |
| 3. | Ligase | 4. | EcoRI |
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DNA fragments are
1. Negatively charged
2. Neutral
3. Either positively or negatively charged depending on their size.
4. Positively charged.
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The correct order of steps in Polymerase Chain Reaction (PCR) is:
1. Denaturation, Extension, Annealing
2. Annealing, Extension, Denaturation
3. Extension, Denaturation, Annealing
4. Denaturation, Annealing, Extension
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
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Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
| 1. | Host DNA is packed into chromosomes |
| 2. | Restriction enzymes are ineffective on host DNA |
| 3. | Host DNA does not have the restriction site for the restriction enzymes. |
| 4. | Restriction site of host DNA is methylated. |
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Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
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