Q. No.The correct order of steps in Polymerase Chain Reaction (PCR) is:
1. Denaturation, Extension, Annealing
2. Annealing, Extension, Denaturation
3. Extension, Denaturation, Annealing
4. Denaturation, Annealing, Extension
1. Denaturation, Extension, Annealing
2. Annealing, Extension, Denaturation
3. Extension, Denaturation, Annealing
4. Denaturation, Annealing, Extension
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
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Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
| 1. | Host DNA is packed into chromosomes |
| 2. | Restriction enzymes are ineffective on host DNA |
| 3. | Host DNA does not have the restriction site for the restriction enzymes. |
| 4. | Restriction site of host DNA is methylated. |
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Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
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Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
| 1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
| 2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
| 3. | Both will select only transformants. |
| 4. | Both will select only recombinants. |
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A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase
If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?
| 1. | Any cell of the human body as the DNA is the same in all cells |
| 2. | Any nucleated cell of the human body |
| 3. | Beta cells of islets of Langerhans of the human pancreas |
| 4. | WBCs |
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