Q. No.
Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
| 1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
| 2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
| 3. | Both will select only transformants. |
| 4. | Both will select only recombinants. |
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A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3. Beta Galactosidase
4. Chloramphenicol acetyl transferase
If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?
| 1. | Any cell of the human body as the DNA is the same in all cells |
| 2. | Any nucleated cell of the human body |
| 3. | Beta cells of islets of Langerhans of the human pancreas |
| 4. | WBCs |
What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
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The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
| 1. | Salmonella typhimurium | 2. | Escherechia coli |
| 3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
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The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
| 1. | depends on a particular or sequence of the main chromosomal DNA |
| 2. | is possible due to promoter sequence present in the insert |
| 3. | cannot occur without telomeric sequences |
| 4. | is independent of the control of chromosomal DNA |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
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