The first type II restriction endonuclease discovered that could cut dsDNA specific site was:

1. EcoRI

2. SmaI

3. Hind II

4. Hind III

Which statement regarding restriction endonucleases is NOT correct?

1. They recognize a specific base sequence in the DNA.

2. They are produced by bacterial cells as a primitive immune system.

3. They digest DNA by removing nucleotides from a free 3' end.

4. They often generate stick ends.

Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?

1. BamHI

2. Psti

3. Clal

4. Pvull

In the screening process during rDNA experiments, clones that metabolize $\beta$-gal turn:

1. Colorless

2. Blue

3. Yellow

4. Green

Microinjection is suitable for :

1. Injecting an ovum into the sperm in IVF

2. Transforming animal cells

3. Injecting very small sized drug particles into neurons

4. Conferring antibiotic resistance to certain strain of bacteria 

Polymerase Chain Reaction is not used in :

1. Confirming presence of a pathogen during early infection

2. Identifying the mutated genes in suspected cancer patients

3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures

4. Detection of the presence of HIV in suspected AIDS patient

The plasmid pBR322 does not contain:

1. An origin of replication site

2. A gene that encodes for restrictor of plasmid copy number

3. Gene for Ampicillin and Streptomycin resistance

4. Sites for many restriction enzymes

Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

The bioreactor shown in the diagram is:

1. Sparged stirred tank type

2. Airlift type

3. Simple stirred tank type

4. Fluidized bed type

What is true about the steps of PCR shown in the diagram given below?

l. Step A occurs at the temperature of 95-98${}^{\mathrm{o}}$ C

ll. Step B occurs at the temperature of 55${}^{\mathrm{o}}$ C

lll. Step C occurs at the temperature of 72${}^{\mathrm{o}}$ C

1. l and ll only

2. l and lll only

3. ll and lll only

4. l, ll and lll