Taq polymerase is used in the polymerase chain reaction because:

1. It replicates DNA faster than other enzymes

2. It is the only enzyme that can replicate DNA invitro

3. It does not require an RNA primer to function

4. It is thermostable 

Subtopic:  Large Scale Production: I | Large Scale Production |
 82%
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Plants are more easily manipulated by genetic engineering than are animals because:

1. Plant genes do not contain introns

2. More vectors are available to transfer rDNA into plant cells

3. Totipotency is a feature generally associated with plant cells and not with animal cells

4. Genes can be inserted into animal cells by microinjection.

Subtopic:  Intro to Agri Applications |
 59%
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Identify the incorrectly matched pair :

1.

 Humulin

First therapeutic rDNA product approved for human use

2.

RNAi

Silencing of mRNA with the help of dsRNA

3.

Rosie

Transgenic sheep producing alpha 1 antitrypsin

4.

Golden rice

Vitamin A enriched rice variety

Subtopic:  Transgenic Microbes & Animals |
 79%
From NCERT
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RNAi is a mechanism to silence genes with the help of 

1. ssRNA

2. dsRNA

3. ssDNA

4. dsDNA

Subtopic:  Interference RNA Tech |
 83%
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To protect tobacco plants against Meloidegyne incognita using RNAi, nematode specific genes were introduced in the plant using:

1. Transposons

2. Retrotransposons

3. Virus with RNA genome 

4. Agrobacterium

Subtopic:  Interference RNA Tech |
 66%
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A permanent cure for ADA deficiency could be:

1. Enzyme replacement therapy
2. Bone marrow transplant
3. Introduction of a functional ADA cDNA into lymphocytes
4. Introduction of gene isolated from marrow cells producing ADA into early embryonic cells
Subtopic:  Gene Therapy |
 84%
From NCERT
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Polymerase Chain Reaction is not used in :

1. Confirming presence of a pathogen during early infection

2. Identifying the mutated genes in suspected cancer patients

3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures

4. Detection of the presence of HIV in suspected AIDS patient

Subtopic:  Molecular Diagnosis |
 67%
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If gene isolate from marrow cells producing ADA is introduced into cells at early embryonic stage, it could be a permanant cure of ADA deficiency because the early embryonic cells :

1. Are more differentiated than the later stage cells

2. Are constantly dividing and do not get differentiated 

3. Are easy to manipulate than the later stage cells

4. Are virtually immortal 

Subtopic:  Gene Therapy |
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To test the safety of polio vaccine, the organisms used are transgenic :

1. Mice

2. Pigs

3. Monkey 

4. Cattle

Subtopic:  Transgenic Microbes & Animals |
 87%
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If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective ?

(1) clonal propagation

(2) cybridization

(3) protoplast fusion

(4) mutant selection

Subtopic:  Intro to Agri Applications |
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