What controls the copy number of linked DNA in a transformed bacterial cell?
1.
Ori
2.
Cloning sites
3.
Selectable marker
4.
Reporter gene
Subtopic: Cloning Vector |
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The fragments created by cutting DNA with restriction endonucleases can be separated by gel electrophoresis. What is incorrect regarding this procedure?
| 1. | The fragments move towards anode. |
| 2. | Agar gel is used for sieving effect. |
| 3. | DNA fragments can be visualized moving in the medium if stained with ethidium bromide followed by exposure to UV radiation. |
| 4. | The larger fragments move farther in the medium. |
Subtopic: Separation and Isolation of DNA fragments |
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The discipline of biotechnology was founded on the breakthrough work of:
| 1. | Bolivar and Rodriquez | 2. | Cohen and Boyer |
| 3. | Nathans and Daniels | 4. | Redi and Spallanzani |
Subtopic: Introduction & History: I | Introduction & History: II | Restriction Enzymes: Historical Background |
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
| 1. | EcoR I | 2. | Hind II |
| 3. | Bam HI | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
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In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Large Scale Production |
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A plasmid contains two genes for resistance to separate antibiotics. One of the two is insertionally inactivated due to insertion of alien DNA.
| I: | The gene that was not inactivated helps in selecting the transformants. |
| II: | The gene that was insertionally inactivated helps in selection of recombinants. |
| 1. | Only I is correct |
| 2. | Only II is correct |
| 3. | Both I and II are correct |
| 4. | I, II and III are correct |
Subtopic: Cloning Vector | Selection of Recombinant Transformants |
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In Polymerase chain reaction:
| 1. | the correct chronological sequence of steps is: Annealing of primer → Denaturation → Extension. |
| 2. | Taq polymerase is an RNA polymerase derived from a fungus. |
| 3. | millions to billions of copies of a specific DNA sample are made. |
| 4. | viral antigens cannot be detected in a given sample. |
Subtopic: Polymerase Chain Reaction: PCR |
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Which of the following statements will be correct regarding restriction endonucleases used in constructing recombinant DNA molecules?
| 1. | They are RNA molecules acting as enzymes. |
| 2. | The enzymes used in such experiments cut dsDNA randomly at any site. |
| 3. | They have been isolated from bacterial cells. |
| 4. | All restriction enzymes are thermostable and hence can be used in PCR procedures. |
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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DNA fragments created by the action of restriction enzymes can be separated by agar gel electrophoresis where:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | larger the fragment size, the farther it moves in the gel |
| II: | the DNA fragments are forced to move towards anode under an electric field |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Separation and Isolation of DNA fragments |
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A ‘continuous culture system’ method usually produces a larger biomass mainly because:
| 1. | the environmental conditions for the growth of the microbes are very easily maintained in such a system |
| 2. | such a system keeps most of the cells in their active/log exponential phase |
| 3. | it is very cheap with respect to other systems and hence cost of production is low |
| 4. | such a system uses larger sized bioreactors than other systems |
Subtopic: Large Scale Production |
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