RNA interference:
I: involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).
II: has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita
 
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect

Subtopic:  Interference RNA Tech |
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To produce human recombinant insulin, Eli Lily:
1. prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains.
2. prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains.
3. used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA.
4. used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells.
Subtopic:  Med Applications & Humulin |
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Which of the following can permanently cure ADA deficiency?
1. Bone marrow transplant
2. Enzyme replacement therapy
3. Introduction of the gene isolated from marrow cells producing ADA at early embryonic stages.
4. Introduction of a functional ADA cDNA (using a retroviral vector) into peripheral lymphocytes.
Subtopic:  Gene Therapy |
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Identify the incorrect statement regarding transgenic animals:
1. α-1 antitrypsin is being produced in a transgenic sheep
2. The first transgenic cow, Rosie, produced human protein-enriched milk (2.4 grams per litre).
3. Transgenic mice are being used to test the safety of the polio vaccine.
4. Over 95 percent of all existing transgenic animals are cattle.
Subtopic:  Transgenic Microbes & Animals |
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GEAC stands for:
1. Genetic Engineering Approval Committee  
2. Green Environment Action Committee
3. Genetic Engineering Appraisal Council
4. Genetic Ethics Approval Committee
Subtopic:  Ethical Issues |
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The term used to refer to the use of bio-resources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is:
1. Bioprospecting
2. Biopiracy
3. Biomagnification
4. Bioaccumulation

Subtopic:  Ethical Issues |
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Identify the incorrectly matched pair:
1. Golden rice Vitamin B12 enriched rice
2. Rosie First transgenic cow
3. Bt Cotton Genetically modified pest resistant plant cotton variety
4. Basmati controversy Biopiracy
Subtopic:  Other GM Crops |
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A chronic respiratory disorder, commonly caused by cigarette smoking but rarely due to deficiency of α-1 antitrypsin enzyme, is:
1. Bronchial asthma 2. Tuberculosis
3. Emphysema 4. Pneumoconiosis
Subtopic:  Transgenic Microbes & Animals |
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ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography

Subtopic:  Interference RNA Tech |
 82%
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NEET - 2022
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In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because: 
1. Genetically engineered lymphocytes are not immortal cells.
2. Retroviral vector is introduced into these lymphocytes.
3. Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages 
4. Lymphocytes from patient's blood are grown in culture, outside the body.
Subtopic:  Gene Therapy |
 76%
From NCERT
NEET - 2022
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