I: | involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). |
II: | has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita |
1. | prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. |
2. | prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains. |
3. | used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA. |
4. | used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells. |
1. | Bone marrow transplant |
2. | Enzyme replacement therapy |
3. | Introduction of the gene isolated from marrow cells producing ADA at early embryonic stages. |
4. | Introduction of a functional ADA cDNA (using a retroviral vector) into peripheral lymphocytes. |
1. | α-1 antitrypsin is being produced in a transgenic sheep |
2. | The first transgenic cow, Rosie, produced human protein-enriched milk (2.4 grams per litre). |
3. | Transgenic mice are being used to test the safety of the polio vaccine. |
4. | Over 95 percent of all existing transgenic animals are cattle. |
1. | Genetic Engineering Approval Committee |
2. | Green Environment Action Committee |
3. | Genetic Engineering Appraisal Council |
4. | Genetic Ethics Approval Committee |
The term used to refer to the use of bio-resources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is:
1. Bioprospecting
2. Biopiracy
3. Biomagnification
4. Bioaccumulation
1. | Golden rice | Vitamin B12 enriched rice |
2. | Rosie | First transgenic cow |
3. | Bt Cotton | Genetically modified pest resistant plant cotton variety |
4. | Basmati controversy | Biopiracy |
1. | Bronchial asthma | 2. | Tuberculosis |
3. | Emphysema | 4. | Pneumoconiosis |
ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography
1. | Genetically engineered lymphocytes are not immortal cells. |
2. | Retroviral vector is introduced into these lymphocytes. |
3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
4. | Lymphocytes from patient's blood are grown in culture, outside the body. |