| 1. | prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them into plasmids of E. coli to produce insulin chains. |
| 2. | prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains. |
| 3. | used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA. |
| 4. | used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells. |
| 1. | Bone marrow transplant |
| 2. | Enzyme replacement therapy |
| 3. | Introduction of the gene isolated from marrow cells producing ADA at early embryonic stages. |
| 4. | Introduction of a functional ADA cDNA (using a retroviral vector) into peripheral lymphocytes. |
| 1. | α-1 antitrypsin is being produced in a transgenic sheep |
| 2. | The first transgenic cow, Rosie, produced human protein-enriched milk (2.4 grams per litre). |
| 3. | Transgenic mice are being used to test the safety of the polio vaccine. |
| 4. | Over 95 percent of all existing transgenic animals are cattle. |
| 1. | Genetic Engineering Approval Committee |
| 2. | Green Environment Action Committee |
| 3. | Genetic Engineering Appraisal Council |
| 4. | Genetic Ethics Approval Committee |
The term used to refer to the use of bio-resources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is:
1. Bioprospecting
2. Biopiracy
3. Biomagnification
4. Bioaccumulation
| 1. | Golden rice | Vitamin B12 enriched rice |
| 2. | Rosie | First transgenic cow |
| 3. | Bt Cotton | Genetically modified pest resistant plant cotton variety |
| 4. | Basmati controversy | Biopiracy |
| 1. | Bronchial asthma | 2. | Tuberculosis |
| 3. | Emphysema | 4. | Pneumoconiosis |
ds RNA can be used during which one of the following?
| 1. | Gene sequencing | 2. | Polymerase Chain Reaction |
| 3. | Gene silencing | 4. | Autoradiography |
| 1. | Genetically engineered lymphocytes are not immortal cells. |
| 2. | Retroviral vector is introduced into these lymphocytes. |
| 3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
| 4. | Lymphocytes from patient's blood are grown in culture, outside the body. |
| (a) | Pro-hormone insulin contain extra stretch of C-peptide |
| (b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
| (c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
| (d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
| (e) | Some patients develop allergic reactions to the foreign insulin. |