(a) | Pro-hormone insulin contain extra stretch of C-peptide |
(b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
(c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
(d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
(e) | Some patients develop allergic reactions to the foreign insulin. |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
1. | (A) is True but (R) is False. |
2. | (A) is False but (R) is True. |
3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
1. | Gene therapy – Use of retrovirus to introduce cDNA into the host cell. |
2. | PCR – Used to detect mutations is genes in suspected cancer patients |
3. | ELISA – Detection of non-proteinecious antigens. |
4. | Probe – A single strand DNA or RNA, tagged with radioactive molecule, will never hybridized with mutated gene |
Assertion(A): | Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with roots of pulse crops. |
Reason(R): | A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated |
In the following questions a statement of assertion (A) is followed by a statement of reason (R).
1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are False. |
Statement I: | Proteins encoded by genes cryIAc and cryllAb control corn borer, that of crylAb control the cotton bollworms |
Statement II: | Bt toxin protein exist as inactive protoxins but once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut |
1. | Human protein \(\alpha\) –1–antitrypsin. |
2. | Human alpha–lactalbumin. |
3. | Human insulin–like growth factor. |
4. | Human enzyme Adenosine Deaminase (ADA). |