The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
Subtopic: Â Process of Biotech |Â Introduction & History |
 74%
From NCERT
NEET - 2022
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Â Polymerase Chain Reaction: PCR |
 67%
From NCERT
NEET - 2022
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c), (d) and (e) only
Subtopic: Â Cloning Vector |Â Tools |
 87%
From NCERT
NEET - 2022
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| Assertion(A): | Insertional inactivation method helps to differentiate recombinant from non-recombinant |
| Reason(R): | Selection of recombinant in this method depends upon inactivation of antibiotics |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Selection of Recombinant Transformants |
From NCERT
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Which of the following is true about Stanley cohen and Herbet Boyer experiment ?
| 1. | They used plasmid of salmonella typhimurium as a vector |
| 2. | They used plasmid of Escherichia coli to obtained antibiotic resistance gene |
| 3. | They used molecular scissor’s to obtained antibiotic resistance gene from the plasmid of salmonella typhimurium |
| 4. | They used DNA ligase to join antibiotic resistance gene obtained from Escherichia coli to the plasmid of salmonella |
Subtopic: Â Restriction Enzymes - Main Enzymes |
From NCERT
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Gene transfer involves some essential stages such as
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
| (i) | inserting recombinant DNA into suitable recipient organism |
| (ii) | isolation of a fragment of DNA |
| (iii) | screening of transformed cells |
| (iv) | integrating DNA fragment into suitable vector |
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
Subtopic: Â Transforming Plant & Animal Cell |
 84%
From NCERT
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Â Tools |Â General Design of an rDNA experiment |
 74%
From NCERT
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Which of the following DNA is used by Agrobacterium tumefaciens to transform normal cells into a tumor?
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
Subtopic: Â Transforming Plant & Animal Cell |
 89%
From NCERT
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Selection of recombinations based on their inability to produce colour in the presence of a chromogenic substrate is
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
Subtopic: Â Cloning Vector |
 89%
From NCERT
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Restriction endonucleases are bacterial enzymes that cut double stranded DNA. These restriction enzymes do not cut their own DNA because
| 1. | The bacterium does not contain the specific recognition sequence |
| 2. | The DNA is packed with histones |
| 3. | The specific recognition sequences are masked with methyl groups |
| 4. | Restriction endonucleases cut viral DNA only |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 82%
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