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Q. No.Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: General Design of an rDNA experiment |
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Given below are two statements one is labelled as Assertion (A) and the other is labelled as Reason (R).
In light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
In light of the above statements, choose the correct answer from the options given below:
| 1. | Both (A) and (R) are true and (R) correctly explains (A). |
| 2. | Both (A) and (R) are true and (R) does not correctly explain (A). |
| 3. | (A) is true, (R) is false. |
| 4. | (A) is false, (R) is true. |
Subtopic: Restriction Enzymes - Main Enzymes |
60%
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NEET - 2022
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Separation and Isolation of DNA fragments |
90%
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NEET - 2022
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Match List-I with List-II :
Choose the correct answer from the options given below:
| List I | List II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
Subtopic: Transforming Plant & Animal Cell |
92%
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NEET - 2022
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The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
Subtopic: General Design of an rDNA experiment |
93%
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NEET - 2022
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic: Cloning Vector |
84%
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NEET - 2022
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Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?
1. Agrobacterium mediated transformation
2. Gene gun
3. 'Disarmed pathogen' vectors
4. Bacteriophages
1. Agrobacterium mediated transformation
2. Gene gun
3. 'Disarmed pathogen' vectors
4. Bacteriophages
Subtopic: Transforming Plant & Animal Cell |
57%
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NEET - 2022
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Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
Choose the most appropriate answer from the options given below :
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Separation and Isolation of DNA fragments |
82%
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NEET - 2022
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
| 1. | Cosmids | 2. | Plasmids |
| 3. | Nucleus | 4. | Nucleoid |
Subtopic: Process of Biotech |
87%
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NEET - 2022
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Complete the analogy with respect to the method of gene transfer and the most suitable host cell for it
Microinjection : Animal cell :: Biolistics:______
Select the correct option:
1. Bacteria
2. Plant cell
3. Bacteriophage
4. Fungal cell
Microinjection : Animal cell :: Biolistics:______
Select the correct option:
1. Bacteria
2. Plant cell
3. Bacteriophage
4. Fungal cell
Subtopic: Transforming Plant & Animal Cell |
93%
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