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Q. No.The enzyme used in PCR:
I:
is a DNA-dependent DNA polymerase
II:
extends the primers in template independent manner
III:
is thermostable
1.
Only I and II are correct
2.
Only I and III are correct
3.
Only II and III are correct
4.
I, II and III are correct
Subtopic: Polymerase Chain Reaction: PCR |
From NCERT
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Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism comes under:
1. Bioprocess engineering
2. Genetic engineering
3. Bioinformatics
4. Biomedical technology
1. Bioprocess engineering
2. Genetic engineering
3. Bioinformatics
4. Biomedical technology
Subtopic: General Design of an rDNA experiment |
90%
From NCERT
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If a plasmid is to be used as a cloning vector, the most important feature which must be present in it is:
| 1. | Origin of replication (Ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Being linear rather than circular |
Subtopic: Cloning Vector |
82%
From NCERT
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What would not be true for a plasmid present in some bacterial cells?
| 1. | It is extrachromosomal DNA |
| 2. | It is circular and double stranded |
| 3. | It gets replicated with the help of ori of chromosomal DNA |
| 4. | It confers characters like resistance to antibiotics to bacterial cells |
Subtopic: Cloning Vector |
63%
From NCERT
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Restriction enzymes produced by some bacteria:
| 1. | cleave phosphodiester bond in the main chromosomal DNA of the bacterium. |
| 2. | cut single stranded DNA at random positions. |
| 3. | prevent the multiplication of bacteriophage in bacteria. |
| 4. | provide resistance against antibiotics. |
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
52%
From NCERT
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Which of the following methods is especially useful for transforming animal cells?
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
Subtopic: Transforming Plant & Animal Cell |
89%
From NCERT
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The DNA fragments separated on agar gel by electrophoresis can be visualised:
| 1. | without staining in the visible light |
| 2. | without staining on exposure to UV light |
| 3. | after staining with polyethylene glycol followed by exposure to IR radiation |
| 4. | after staining with ethidium bromide followed by exposure to UV radiation |
Subtopic: Separation and Isolation of DNA fragments |
94%
From NCERT
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Tetracycline resistance gene in the vector pBR322 carries the recognition sequence for:
1. PstI
2. PvuII
3. BamH I
4. EcoR I
1. PstI
2. PvuII
3. BamH I
4. EcoR I
Subtopic: Cloning Vector |
88%
From NCERT
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Restriction Enzymes: Historical Background | Tools |
91%
From NCERT
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The number of times the recognition sequence of EcoRI is expected to appear in a dsDNA of 10,000 bp length will be:
1. 1
2. 2
3. 4
4. 8
1. 1
2. 2
3. 4
4. 8
Subtopic: Restriction Enzymes - Main Enzymes |
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