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Q. No.If a plasmid is to be used as a cloning vector, the most important feature which must be present in it is:
1.
Origin of replication (Ori)
2.
Presence of a selectable marker
3.
Presence of sites for restriction endonuclease
4.
Being linear rather than circular
Subtopic: Cloning Vector |
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What would not be true for a plasmid present in some bacterial cells?
| 1. | It is extrachromosomal DNA |
| 2. | It is circular and double stranded |
| 3. | It gets replicated with the help of ori of chromosomal DNA |
| 4. | It confers characters like resistance to antibiotics to bacterial cells |
Subtopic: Cloning Vector |
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Restriction enzymes produced by some bacteria:
| 1. | cleave phosphodiester bond in the main chromosomal DNA of the bacterium. |
| 2. | cut single stranded DNA at random positions. |
| 3. | prevent the multiplication of bacteriophage in bacteria. |
| 4. | provide resistance against antibiotics. |
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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Which of the following methods is especially useful for transforming animal cells?
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
Subtopic: Transforming Plant & Animal Cell |
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The DNA fragments separated on agar gel by electrophoresis can be visualised:
| 1. | without staining in the visible light |
| 2. | without staining on exposure to UV light |
| 3. | after staining with polyethylene glycol followed by exposure to IR radiation |
| 4. | after staining with ethidium bromide followed by exposure to UV radiation |
Subtopic: Separation and Isolation of DNA fragments |
94%
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Tetracycline resistance gene in the vector pBR322 carries the recognition sequence for:
1. PstI
2. PvuII
3. BamH I
4. EcoR I
1. PstI
2. PvuII
3. BamH I
4. EcoR I
Subtopic: Cloning Vector |
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Restriction Enzymes: Historical Background | Tools |
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The number of times the recognition sequence of EcoRI is expected to appear in a dsDNA of 10,000 bp length will be:
1. 1
2. 2
3. 4
4. 8
1. 1
2. 2
3. 4
4. 8
Subtopic: Restriction Enzymes - Main Enzymes |
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A plasmid has a single recognition sequence for EcoRI. When cut with this enzyme, the number of resultant fragments will be:
1. 1
2. 2
3. 3
4. 4
Subtopic: Cloning Vector |
From NCERT
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The most critical requirement for any DNA molecule to be able to act as a cloning vector during rDNA procedures will be:
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
Subtopic: Cloning Vector |
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