A plasmid has a single recognition sequence for EcoRI. When cut with this enzyme, the number of resultant fragments will be:
1. 1
2. 2
3. 3
4. 4
a: | DNA fragments are negatively charged molecules and can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. |
b: | The separation of DNA fragments is possible because they differ in size. |
c: | Smaller the size of the DNA fragment, shorter the distance it travels through agarose gel. |
d: | Pure DNA can be visualized directly by exposing it to UV radiation without any staining. |
1. | Polysaccharides | 2. | RNA |
3. | DNA | 4. | Histones |
A. | Insertion of recombinant DNA into the host cell. |
B. | Cutting of DNA at specific location by restriction enzyme. |
C. | Isolation of desired DNA fragment. |
D. | Amplification of gene of interest using PCR. |
1. | B, D, A, C | 2. | B, C, D, A |
3. | C, A, B, D | 4. | C, B, D, A |
1. | It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. |
2. | 'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells. |
3. | It transforms normal plant cells into tumor cells |
4. | It delivers 'T-DNA' into plant cell |