Which of the following genes do not occur naturally in living organisms?

(1) Bt genes

(2) RNAi genes

(3) Cry genes

(4) Endogeneous cytoplasmic defense genes

What is true about Bt toxin?

(1) Bt protein exists as active toxin in the Bacillus.

(2) The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.

(3) The concerned Bacillus has antitoxins.

(4) The inactive protoxin gets converted into active form in the insect gut.

An example of gene therapy is

(1) production of injectable hepatitis B vaccine

(2) production of vaccines in food crops like potatoes which can be eaten

(3) introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)

(4) production of test tube babies by artificial insemination and implantation of fertilised eggs.

Adenosine deaminase (ADA deficiency) could be permanently cure, if the gene isolated from marrow cells producing ADA is introduced into cell at

(1) early embryonic stages

(2) Late embryonic stages

(3) early childhood

(4) None of the above

The first clinical gene therapy was given for treating

(1) diabetes mellitus

(2) chickenpox

(3) rheumatoid arthritis

(4) adenosine deaminase deficiency

The source of taq polymerase used in PCR is a

(1) thermophilic fungus

(2) mesophilic fungus

(3) thermophilic bacterium

(4) halophilic bacterium

The protein products of the following Bt toxin genes cry I Ac and cry II Ab are responsible for controlling

(1) bollworm

(2) roundworm

(3) moth

(4) fruit fly

Three critical research areas of biotechnology are:

I. Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme

II. Multiple Ovulation Transfer Technology (MOET)

III. Creating optimal conditions through engineering for a catalyst to act.

IV. Downstream processing technologies

(1) I, II, III, IV      

(2) I, III, IV          

(3) I, II                 

(4) II and IV

Bt toxin is: 

(1) Intracellular lipids                      

(2) Intracellular crystalline protein

(3) Extracellular crystalline protein    

(4) Intracellular polysaccharide

Bt toxin is harmful to insects like:

(1) Lepidoterans (tobacco budworm, armyworms)

(2) Coleopterans (beetles)

(3) Dipterans (flies and mosquito)

(4) All are correct