The source of taq polymerase used in PCR is a

(1) thermophilic fungus

(2) mesophilic fungus

(3) thermophilic bacterium

(4) halophilic bacterium

The protein products of the following Bt toxin genes cry I Ac and cry II Ab are responsible for controlling

(1) bollworm

(2) roundworm

(3) moth

(4) fruit fly

Three critical research areas of biotechnology are:

I. Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme

II. Multiple Ovulation Transfer Technology (MOET)

III. Creating optimal conditions through engineering for a catalyst to act.

IV. Downstream processing technologies

(1) I, II, III, IV      

(2) I, III, IV          

(3) I, II                 

(4) II and IV

Bt toxin is: 

(1) Intracellular lipids                      

(2) Intracellular crystalline protein

(3) Extracellular crystalline protein    

(4) Intracellular polysaccharide

Bt toxin is harmful to insects like:

(1) Lepidoterans (tobacco budworm, armyworms)

(2) Coleopterans (beetles)

(3) Dipterans (flies and mosquito)

(4) All are correct

Antisense RNA is:

(1) RNA that makes opposite sense

(2) RNA that investigators find confusing

(3) The non-coding strand of DNA molecule

(4) RNA that is complementary to certain mRNA

Which of the following statements is false?

1. Only I and II

2. Only III and IV

3. Only III

4. Only VI

Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering? 

(1) Splitting A and B polypeptide chains

(2) Addition of C-peptide to pro-insulin

(3) Getting insulin assembled into mature form

(4) Removal of C-peptide from active insulin

Which technique would you expect to be completely curative in SCID?

(1) Gene therapy in adult stage

(2) Gene therapy in embryonic stage

(3) Bone marrow transplantation

(4) Enzyme replacement therapy

For effective treatment of a disease: 

(1) Early diagnosis is needed but understanding of its pathophysiology is not needed

(2) Early diagnosis is not needed but understanding of its pathophysiology is needed

(3) Early diagnosis and understanding of its pathophysiology are needed

(4) Neither early diagnosis nor understanding of its pathophysiology is needed