Select the correct order of processing of PCR.

(1) Extension, primer annealing, denaturation

(2) Denaturation, primer annealing, extension

(3) Denaturation, extension, primer annealing

(4) Primer annealing, denaturation, extension

Plasmid vector in DNA recombinant technology means

1. a virus that transfers gene to bacteria

2. extra-chromosomal autonomously replicating circular DNA

3. sticky end of DNA

4. any fragment of DNA carrying desirable gene

Which organism is used to transfer T-DNA?

(1) Streplomyces hygroscopicus

(2) Agrobacterium tumefaciens

(3) Salmonella typhi

(4) Escherichia coli

Match the following column I with column II.

Column I                                      Column II

A. Agrostology                              1. Earth

B. Smallest flowering plant            2. Genetic engineering

C. Tectonic                                   3.Migration

D. Ecesis                                      4.Wolffia

E. Euphanix                                  5.Grass

Codes

       A     B     C     D     E

(a)   5     4     1     3     2   

(b)   1     2     3     4     5

(c)   2     3     1     5     4

(d)   5     4     3     2     1

Biolistic (gene gun) is suitable for

1. disarming pathogen vectors

2. transformation of plant cell

3. constructing recombinant DNA by joining with vectors

4. DNA fingerprinting

A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(1) b, a, c, d

(2) a, b, c, d

(3) c, b, a, d

(4) b, a, d, c

Which of the following DNA sequences qualifies to be designated as a palindrome?

(1) 5 - GACCAG - 3’ in one strand

(2) 3’- GACCAG - 5’ in one strand

(3) 5’- GACGAG- 3’ 3’- CTGGTC - 5’

(4) 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

pBR_322, which is frequently used as a vector for cloning gene in E. coli is a/an

1. original bacterial plasmid

2. modified bacterial plasmid

3. viral genome

4. transposon

The thermostable enzyme Taq polymerase used in PCR technique was discovered by

(1)Alec Jeffrey

(2)Karry Mullis

(3)Temin

(4)Baltimore

To be useful in genetic engineering a vector must have

1. An origin for replication
2. Low copy number
3. Low rates of multiplication
4. Many cloning sites for the same enzyme