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Q. No.The accessibility of promoter regions of prokaryotic DNA is in many cases regulated by the interaction of
proteins with sequences termed:
1. Enhancers
2. Silencers
3. Operators
4. Terminators
Subtopic: Gene Regulation: Lac Operon |
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The first to elucidate a transcriptionally regulated system of gene expression were:
1. Jacob and Monod
2. Nirenberg and Khorana
3. Tijo and Levan
4. Beadle and Tatum
1. Jacob and Monod
2. Nirenberg and Khorana
3. Tijo and Levan
4. Beadle and Tatum
Subtopic: Transcription:II |
81%
Level 1: 80%+
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Which of the following statements will be true regarding the effect of mutations in the components of the lac operon of E. coli [assume mutations will lead to loss of function in the affected gene]?
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | A mutation in the i gene will lead to a stoppage of transcription of the operon. |
| II: | A mutation in the z gene will lead to an inability in E .coli to hydrolyze the disaccharide, lactose. |
| III: | A mutation in the y gene will lead to an inability in E. coli to pick up lactose from its surroundings. |
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Gene Regulation: Lac Operon |
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Which of the following is synthesised all-the-time – constitutively in E .coli?
1. repressor of the lac operon
2. beta galactosidase
3. permease
4. transacetylase
1. repressor of the lac operon
2. beta galactosidase
3. permease
4. transacetylase
Subtopic: Gene Regulation: Lac Operon |
72%
Level 2: 60%+
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The important goals of HGP included all of the following, except:
| 1. | Identify all the approximately 20,000–25,000 genes in human DNA |
| 2. | Determine the sequences of the 3 billion chemical base pairs that make up human DNA |
| 3. | Develop technology for cloning of human beings |
| 4. | Address the ethical, legal, and social issues (ELSI) that may arise from the project. |
Subtopic: Human Genome Project |
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Level 1: 80%+
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Many non-human model organisms have also been sequenced even before sequencing of human genome where:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | Caenorhabditis elegans is a pathogenic nematode |
| II: | Arabidopsis thaliana is a fungus |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Human Genome Project |
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Level 3: 35%-60%
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The methodology used in human genome sequencing that took the blind approach of simply sequencing the
whole set of genome that contained all the coding and non-coding sequence, and later assigning different
regions in the sequence with functions, is called as:
1. Expressed sequence tag
2. Sequence annotation
3. Chain termination
4. Physical mapping
whole set of genome that contained all the coding and non-coding sequence, and later assigning different
regions in the sequence with functions, is called as:
1. Expressed sequence tag
2. Sequence annotation
3. Chain termination
4. Physical mapping
Subtopic: Human Genome Project |
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Level 1: 80%+
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The last of the 24 human chromosomes – 22 autosomes and X and Y – to be sequenced was:
1. Chromosome 1
2. the X chromosome
3. the Y chromosome
4. Chromosome 22
1. Chromosome 1
2. the X chromosome
3. the Y chromosome
4. Chromosome 22
Subtopic: Human Genome Project |
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Human Genome Project |
87%
Level 1: 80%+
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Salient observations drawn from the human genome project include all of the following, except:
| 1. | The human genome contains 3164.7 million bp. |
| 2. | The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. |
| 3. | The total number of genes is estimated at 80,000 to 1,40,000 genes. |
| 4. | Almost all (99.9 per cent) nucleotide bases are exactly the same in all people. |
Subtopic: Human Genome Project |
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