Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

If I want to check the expression of my gene, then I should use

1.      Vectors supporting high copy number

2.      Vectors supporting low copy number

3.      No need to bother about copy number

4.     Vectors with strong Ori

Selectable marker helps

1.      To identify transformants

2.      To identify recombinants

3.      To identify non- recombinants

4.      To eliminate transformants

The normal E. coli cells have

1.      Kanamycin

2.      Tetracycline

3.      Ampicillin

4.     No resistance

For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

1.      Non- recombinant

2.      Recombinant

3.      Transformants

4.     Non-transformants

Insertional inactivation results in

1.      Non-recombinants

2.      Recombinants

3.      Transformants

4.     Non- transformants

Which of the following options is correct about Agrobacterium tumifaciens?

1.     It is a pathogen of angiosperms

2.     It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

3.     Its Ti plasmid is now modified into an expression vector

4.     Its Ti plasmid is modified into a cloning vector which is no more pathogenic

In order to make the host cell competent

Divalent cations such as calcium are used-

1.      The divalent ions have to be in a specific concentration

2.      They cause the DNA uptake by the cell

3.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

4.     All of the above

The process of competent cell formation include

1.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

2.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

3.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

4.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA