1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
The process of separation and purification of expressed protein before marketing is called:
1. Downstream processing
2. Bioprocessing
3. Post-product processing
4. Upstream processing
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. | Eco Rl | 2. | taq polymerase |
3. | polymerase lll | 4. | ligase |
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
1. | Vector | 2. | Plasmid |
3. | Structural gene | 4. | Selectable marker |
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
1. | The smaller the fragment size, the farther it moves |
2. | Positively charged fragments move to farther end |
3. | Negatively charged fragments do not move |
4. | The larger the fragment size, the farther it moves |
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water