For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:

1. Telomeric sequence 2. Multiple cloning sites
3. Ori 4. Selectable marker

Subtopic:  Cloning Vector |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:

1. EcoRI 2. HindII
3. SmaI 4. BamHI
Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
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Which of the following cannot be used as a vector in rDNA technology?

1.Plasmid2.Phage DNA
3.Bacterium4.YAC

Subtopic:  Tools |
 59%
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The following palindrome is recognized by the restriction enzyme:

 

1.  BamHI 2.  EcoRI
3.  HindII 4.  PstI
Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
 84%
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?

1.  Plastid  2.  Bacteriophages
3.  BAC 4.   Plasmid
Subtopic:  Cloning Vector |
 66%
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pBR322 does not contain the site for:

1.  Pvu I 2. BamH I
3.  Sma I 4. EcoR I
Subtopic:  Cloning Vector |
 82%
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A method used only for transforming animal cells is:

1. Biolistics 2. Microinjection
3. Use of virus 4. Agrobacterium mediation
Subtopic:  Host & Desired DNA |
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Lysozyme should be used when isolating DNA in a pure form from:

1. A bacterial cell 2. A fungal cell
3. A plant cell 4. An animal cell
Subtopic:  Process of Biotech | Restriction Enzymes - Main Enzymes | General Design of an rDNA experiment |
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In recombinant DNA experiments, a vector:

1. carries DNA into a new cell 
2. links together newly joined fragments of DN
3. makes millions of copies of a specific segment of DNA
4. separates fragments of DNA by their length and electrical charges
Subtopic:  Cloning Vector |
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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

1. Lipase 2. Protease
3. Exonuclease 4. Endonuclease

Subtopic:  Restriction Enzymes - Main Enzymes |
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