Q. No.The cutting of DNA at specific locations became possible with the discovery of
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
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Which of the following is not a feature of the plasmids?
1. Independent replication
2. Circular structure
3. Transferable
4. Single-stranded
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The Taq polymerase enzyme is obtained from:
1. Thermus aquaticus
2. Thiobacillus ferrooxidans
3. Bacillus subtilis
4. Pseudomonas putida
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Which of the following is not a component of downstream processing?
1. Purification
2. Preservation
3. Expression
4. Separation
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
| 1. | Taq polymerase | 2. | Polymerase III |
| 3. | Ligase | 4. | EcoRI |
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DNA fragments are
1. Negatively charged
2. Neutral
3. Either positively or negatively charged depending on their size.
4. Positively charged.
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The correct order of steps in Polymerase Chain Reaction (PCR) is:
1. Denaturation, Extension, Annealing
2. Annealing, Extension, Denaturation
3. Extension, Denaturation, Annealing
4. Denaturation, Annealing, Extension
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
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