1. | For a cell to divide, it must first replicate its DNA. |
2. | DNA replication is an all-or-none process; once replication begins, it proceeds to completion. |
3. | DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell). |
4. | Once replication is complete, it does not occur again in the same cell cycle. |
1. | Chromosomal DNA | 2. | Complementary DNA |
3. | F plasmid | 4. | Ti plasmid |
Column I | Column II | ||
A. | Lysozyme | P. | Fungal cell |
B. | Cellulase | Q. | Plant cell |
C. | Chitinase | R. | Bacterial cell |
A | B | C | |
1. | P | Q | R |
2. | P | R | Q |
3. | R | P | Q |
4. | R | Q | P |
1. | Salvador Luria, Jean Weigle and Giuseppe Bertani |
2. | Nirenberg, Matthaei and Korana |
3. | Paul Berg, Stanley Cohen and Herbert Boyer |
4. | Werner Arber, Daniel Nathans, and Hamilton O. Smith |
1. | 256 | 2. | 256,000 |
3. | 1 million | 4. | 1 billion |
1. | DNA ligase | cutting DNA, creating sticky ends of restriction fragments |
2. | DNA polymerase | polymerase chain reaction to amplify sections of DNA |
3. | Electrophoresis | separation of DNA fragments |
4. | Reverse transcriptase | production of cDNA from mRNA |
I: | Agar Gel electrophoresis is useful in analysis of DNA. |
II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | I is correct but II is incorrect |
4. | Both I and II are incorrect |