Consider the two statements:
I: Agar Gel electrophoresis is useful in analysis of DNA.
II: DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole.
 
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect

Subtopic:  Separation and Isolation of DNA fragments |
 71%
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Consider the given two statements:
I. It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II. When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic:  Cloning Vector |
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Bacterial cells synthesize restriction endonucleases because restriction endonucleases help the bacterial cells:
1.    grow readily in medium containing antibodies.
2.    protect themselves from viral infection.
3.    open up the DNA in their chromosomes so that transcription is easier.
4.    take up foreign DNA from their environment and combine it into their genome.
 
Subtopic:  Introduction & History: I | Introduction & History: II | Restriction Enzymes - Main Enzymes |
 63%
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Consider the following two statements: 
I:  Plasmid can be used as a vector in recombinant DNA technology experiments.
II:  Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria.
 
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic:  Tools: Vectors: I | Cloning Vector |
 54%
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What is true regarding Downstream Processing?
I: This stage is after the biosynthetic stage.
II: The processes include separation and purification.
 
1. Only I 2. Only II
3. Both I and II 4. Neither I nor II
Subtopic:  Large Scale Production |
 89%
From NCERT
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Identify the correct statement:
1. Agrobacterium tumefaciens is able to transform large number of monocot plants.
2. Micro-injection is the only way to transform animal cells.
3. Competence in bacterial cells can be induced by trivalent anions like phosphates.
4. Biolistics or gene gun is a suitable method to transform plant cells.
Subtopic:  Transforming Plant & Animal Cell |
 83%
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What controls the copy number of linked DNA in a transformed bacterial cell?
1. Ori 2. Cloning sites
3. Selectable marker 4. Reporter gene
Subtopic:  Cloning Vector |
 88%
From NCERT
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The fragments created by cutting DNA with restriction endonucleases can be separated by gel electrophoresis. What is incorrect regarding this procedure?
1. The fragments move towards anode.
2. Agar gel is used for sieving effect.
3. DNA fragments can be visualized moving in the medium if stained with ethidium bromide followed by exposure to UV radiation.
4. The larger fragments move farther in the medium.
Subtopic:  Separation and Isolation of DNA fragments |
 87%
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The discipline of biotechnology was founded on the breakthrough work of:
1. Bolivar and Rodriquez 2. Cohen and Boyer
3. Nathans and Daniels 4. Redi and Spallanzani
Subtopic:  Introduction & History: I | Introduction & History: II | Restriction Enzymes: Historical Background |
 89%
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
1. EcoR I 2. Hind II
3. Bam HI 4. Sma I
Subtopic:  Restriction Enzymes - Main Enzymes |
 90%
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