For their work in the discovery and characterization of restriction enzymes, the 1978 Nobel Prize for Physiology or Medicine was awarded to:
1.
Salvador Luria, Jean Weigle and Giuseppe Bertani
2.
Nirenberg, Matthaei and Korana
3.
Paul Berg, Stanley Cohen and Herbert Boyer
4.
Werner Arber, Daniel Nathans, and Hamilton O. Smith
Subtopic: Â Restriction Enzymes: Historical Background |
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Beginning with a single template DNA, a PCR reaction is allowed to continue for 30 cycles. The number of copies of the template DNA at the end is expected to be about:
| 1. | 256 | 2. | 256,000 |
| 3. | 1 million | 4. | 1 billion |
Subtopic: Â Polymerase Chain Reaction: PCR |
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PCR does not require:
1. A thermostable DNA polymerase
2. Dideoxy-dNTPs (ddNTPs)
3. Template DNA
4. Primers
1. A thermostable DNA polymerase
2. Dideoxy-dNTPs (ddNTPs)
3. Template DNA
4. Primers
Subtopic: Â Polymerase Chain Reaction: PCR |
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Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
| 1. | DNA ligase | cutting DNA, creating sticky ends of restriction fragments |
| 2. | DNA polymerase | polymerase chain reaction to amplify sections of DNA |
| 3. | Electrophoresis | separation of DNA fragments |
| 4. | Reverse transcriptase | production of cDNA from mRNA |
Subtopic: Â Tools |
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The source of the reverse transcriptase used in recombinant DNA technology, is a:
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
Subtopic: Â General Design of an rDNA experiment |
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Consider the two statements:
| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Separation and Isolation of DNA fragments |
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Consider the given two statements:
I. It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II. When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
I. It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II. When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Â Cloning Vector |
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Bacterial cells synthesize restriction endonucleases because restriction endonucleases help the bacterial cells:
1. grow readily in medium containing antibodies.
2. protect themselves from viral infection.
3. open up the DNA in their chromosomes so that transcription is easier.
4. take up foreign DNA from their environment and combine it into their genome.
1. grow readily in medium containing antibodies.
2. protect themselves from viral infection.
3. open up the DNA in their chromosomes so that transcription is easier.
4. take up foreign DNA from their environment and combine it into their genome.
Subtopic: Â Introduction & History: I |Â Introduction & History: II |Â Restriction Enzymes - Main Enzymes |
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Consider the following two statements:
| I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
| II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Tools: Vectors: I |Â Cloning Vector |
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What is true regarding Downstream Processing?
| I: | This stage is after the biosynthetic stage. |
| II: | The processes include separation and purification. |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Â Large Scale Production |
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