What is incorrect regarding DNA replication?
1.
For a cell to divide, it must first replicate its DNA.
2.
DNA replication is an all-or-none process; once replication begins, it proceeds to completion.
3.
DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell).
4.
Once replication is complete, it does not occur again in the same cell cycle.
Subtopic: Â Polymerase Chain Reaction: PCR |
 75%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Agrobacterium tumefaciens infects the plant through its:
| 1. | Chromosomal DNA | 2. | Complementary DNA |
| 3. | F plasmid | 4. | Ti plasmid |
Subtopic: Â Transforming Plant & Animal Cell |
 93%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Codes:
| Column I | Column II | ||
| A. | Lysozyme | P. | Fungal cell |
| B. | Cellulase | Q. | Plant cell |
| C. | Chitinase | R. | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
Subtopic: Â Host & Desired DNA |
 92%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
For their work in the discovery and characterization of restriction enzymes, the 1978 Nobel Prize for Physiology or Medicine was awarded to:
| 1. | Salvador Luria, Jean Weigle and Giuseppe Bertani |
| 2. | Nirenberg, Matthaei and Korana |
| 3. | Paul Berg, Stanley Cohen and Herbert Boyer |
| 4. | Werner Arber, Daniel Nathans, and Hamilton O. Smith |
Subtopic: Â Restriction Enzymes: Historical Background |
Level 4: Below 35%
Please attempt this question first.
Hints
Beginning with a single template DNA, a PCR reaction is allowed to continue for 30 cycles. The number of copies of the template DNA at the end is expected to be about:
| 1. | 256 | 2. | 256,000 |
| 3. | 1 million | 4. | 1 billion |
Subtopic: Â Polymerase Chain Reaction: PCR |
 88%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
PCR does not require:
1. A thermostable DNA polymerase
2. Dideoxy-dNTPs (ddNTPs)
3. Template DNA
4. Primers
1. A thermostable DNA polymerase
2. Dideoxy-dNTPs (ddNTPs)
3. Template DNA
4. Primers
Subtopic: Â Polymerase Chain Reaction: PCR |
 84%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
| 1. | DNA ligase | cutting DNA, creating sticky ends of restriction fragments |
| 2. | DNA polymerase | polymerase chain reaction to amplify sections of DNA |
| 3. | Electrophoresis | separation of DNA fragments |
| 4. | Reverse transcriptase | production of cDNA from mRNA |
Subtopic: Â Tools |
 86%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
The source of the reverse transcriptase used in recombinant DNA technology, is a:
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
Subtopic: Â General Design of an rDNA experiment |
 84%
Level 1: 80%+
Please attempt this question first.
Hints
Consider the two statements:
| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Separation and Isolation of DNA fragments |
 71%
Level 2: 60%+
Hints
Consider the given two statements:
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
| I. | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene. |
| II. | When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors. |
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
© 2025 GoodEd Technologies Pvt. Ltd.