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Q. No.ELISA works on the principle of:
1.
antigen-antibody interaction
2.
radioactive probe tagging
3.
culture of microbe in an artificial medium
4.
histopathology
Subtopic: Molecular Diagnosis |
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Identify the incorrect statement regarding Bacillus thuringiensis?
| 1. | forms protein crystals during a particular phase of their growth which contains a toxic insecticidal protein. |
| 2. | The Bt toxin protein exists as an inactive protoxin that is converted into an active form by an alkaline pH in the insect's gut. |
| 3. | Activated toxin binds to the surface of midgut epithelial cells, creates pores, causes cell swelling and lysis and eventually death of the insect. |
| 4. | Proteins encoded by the genes cryIAc and cryIIAb control the corn borer, that of cryIAb controls cotton bollworms. |
Subtopic: Bt Crops |
83%
From NCERT
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RNA interference:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). |
| II: | has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Interference RNA Tech |
92%
From NCERT
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To produce human recombinant insulin, Eli Lily:
| 1. | prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. |
| 2. | prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains. |
| 3. | used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA. |
| 4. | used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells. |
Subtopic: Med Applications & Humulin |
92%
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Which of the following can permanently cure ADA deficiency?
| 1. | Bone marrow transplant |
| 2. | Enzyme replacement therapy |
| 3. | Introduction of the gene isolated from marrow cells producing ADA at early embryonic stages. |
| 4. | Introduction of a functional ADA cDNA (using a retroviral vector) into peripheral lymphocytes. |
Subtopic: Gene Therapy |
91%
From NCERT
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Identify the incorrect statement regarding transgenic animals:
| 1. | α-1 antitrypsin is being produced in a transgenic sheep |
| 2. | The first transgenic cow, Rosie, produced human protein-enriched milk (2.4 grams per litre). |
| 3. | Transgenic mice are being used to test the safety of the polio vaccine. |
| 4. | Over 95 percent of all existing transgenic animals are cattle. |
Subtopic: Transgenic Microbes & Animals |
83%
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GEAC stands for:
| 1. | Genetic Engineering Approval Committee |
| 2. | Green Environment Action Committee |
| 3. | Genetic Engineering Appraisal Council |
| 4. | Genetic Ethics Approval Committee |
Subtopic: Ethical Issues |
89%
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The term used to refer to the use of bio-resources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is:
1. Bioprospecting
2. Biopiracy
3. Biomagnification
4. Bioaccumulation
Subtopic: Ethical Issues |
95%
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Identify the incorrectly matched pair:
| 1. | Golden rice | Vitamin B12 enriched rice |
| 2. | Rosie | First transgenic cow |
| 3. | Bt Cotton | Genetically modified pest resistant plant cotton variety |
| 4. | Basmati controversy | Biopiracy |
Subtopic: Other GM Crops |
83%
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A chronic respiratory disorder, commonly caused by cigarette smoking but rarely due to deficiency of α-1 antitrypsin enzyme, is:
| 1. | Bronchial asthma | 2. | Tuberculosis |
| 3. | Emphysema | 4. | Pneumoconiosis |
Subtopic: Transgenic Microbes & Animals |
94%
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