Q. No.Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
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Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosomes
2. Yeast artificial chromosomes
3. Plasmid
4. Cosmid
The cutting of DNA at specific locations became possible with the discovery of
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
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Which of the following is not a feature of the plasmids?
1. Independent replication
2. Circular structure
3. Transferable
4. Single-stranded
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The Taq polymerase enzyme is obtained from:
1. Thermus aquaticus
2. Thiobacillus ferrooxidans
3. Bacillus subtilis
4. Pseudomonas putida
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Which of the following is not a component of downstream processing?
1. Purification
2. Preservation
3. Expression
4. Separation
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
| 1. | Taq polymerase | 2. | Polymerase III |
| 3. | Ligase | 4. | EcoRI |
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DNA fragments are
1. Negatively charged
2. Neutral
3. Either positively or negatively charged depending on their size.
4. Positively charged.
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The correct order of steps in Polymerase Chain Reaction (PCR) is:
1. Denaturation, Extension, Annealing
2. Annealing, Extension, Denaturation
3. Extension, Denaturation, Annealing
4. Denaturation, Annealing, Extension
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
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