Identify the incorrect statement:
1. PCR is now routinely used to detect HIV in suspected AIDS patients. 
2. PCR is being used to detect mutations in genes in suspected cancer patients.
3. ELISA is based on the principle of antigen-antibody interaction.
4. ELISA does not detect antibodies and can detect only antigens.

Subtopic:  Molecular Diagnosis |
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The first transgenic cow produced:
1. enzyme to treat emphysema
2. human protein enriched milk
3. enzyme to treat phenylketonuria
4. organs for xenotransplantation
Subtopic:  Transgenic Microbes & Animals |
 93%
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Biopiracy is:
1. the exploration of natural sources for small molecules, macromolecules and biochemical and genetic information that could be developed into commercially valuable products.
2. exploitation of a region's biological resources or indigenous knowledge unethically and without providing fair compensation.
3. the use of living organisms, like microbes and bacteria, in the removal of contaminants, pollutants, and toxins from soil, water, and other environments
4. the process of developing a crop with bioavailable micronutrients in its edible parts.
Subtopic:  Ethical Issues |
 87%
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Consider the given two statements:
I. The first clinical gene therapy was given to a girl with adenosine deaminase deficiency
II. The first recombinant therapeutic product approved for human use was Human Insulin
1. Only I is correct 2. Only II is correct
3. Both I and II are correct 4. Both I and II are incorrect
Subtopic:  Gene Therapy |
 89%
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India successfully protected itself against biopiracy in a case where in late 1997, the United States Patent and Trademark Office granted a patent to:
1. Co-667
2. Sharbati Sonora
3. Lerma Rojo
4. Basmati
Subtopic:  Ethical Issues |
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ELISA works on the principle of:
 
1. antigen-antibody interaction
2. radioactive probe tagging
3. culture of microbe in an artificial medium
4. histopathology
Subtopic:  Molecular Diagnosis |
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Identify the incorrect statement regarding Bacillus thuringiensis?
1. forms protein crystals during a particular phase of their growth which contains a toxic insecticidal protein.
2. The Bt toxin protein exists as an inactive protoxin that is converted into an active form by an alkaline pH in the insect's gut.
3. Activated toxin binds to the surface of midgut epithelial cells, creates pores, causes cell swelling and lysis and eventually death of the insect.
4. Proteins encoded by the genes cryIAc and cryIIAb control the corn borer, that of cryIAb controls cotton bollworms.
Subtopic:  Bt Crops |
 83%
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RNA interference:
I: involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).
II: has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita
 
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic:  Interference RNA Tech |
 92%
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To produce human recombinant insulin, Eli Lily:
1. prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains.
2. prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains.
3. used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA.
4. used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells.
Subtopic:  Med Applications & Humulin |
 92%
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Which of the following can permanently cure ADA deficiency?
1. Bone marrow transplant
2. Enzyme replacement therapy
3. Introduction of the gene isolated from marrow cells producing ADA at early embryonic stages.
4. Introduction of a functional ADA cDNA (using a retroviral vector) into peripheral lymphocytes.
Subtopic:  Gene Therapy |
 91%
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