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Q. No.Read the following statements and choose the correct option.
Statement (A):
In gel electrophoresis, the most commonly used matrix is agar.
Statement (B):
Agar is a natural polymer extracted from sea weeds.
1.
Both statements (A) and (B) are correct
2.
Both statements (A) and (B) are incorrect
3.
Only statement (A) is correct
4.
Only statement (B) is correct
Subtopic: Separation and Isolation of DNA fragments |
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How many statements given below are correct with respect to plasmids?
Select the correct option.
1. Three
2. Four
3. Two
4. One
| a. | They float freely in the cytoplasm of certain bacterial cells. |
| b. | They have the ability to replicate, independent of the genomic DNA. |
| c. | Herbert Boyer developed a method of removing and reinserting them in the cells. |
| d. | They are circular ssDNA molecules. |
1. Three
2. Four
3. Two
4. One
Subtopic: Introduction & History |
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Which of the following can act as selectable markers in a cloning vector?
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
Subtopic: Cloning Vector |
86%
From NCERT
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A mixture containing DNA fragments, p, q, r and s with size of p + r = q, p > r and s > q, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be
1. q, s, p, r
2. s, q, p, r
3. r, p, q, s
4. p, r, s, q
1. q, s, p, r
2. s, q, p, r
3. r, p, q, s
4. p, r, s, q
Subtopic: Separation and Isolation of DNA fragments |
67%
From NCERT
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Hints
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Match column-I with column-II and choose the correct option.
1. a(ii), b(iii), c(iv), d(i)
2. a(ii), b(i), c(iii), d(iv)
3. a(i), b(ii), c(iii), d(iv)
4. a(ii), b(i), c(iv), d(iii)
| Column-I | Column-II | ||
| 1. | Reverse transcriptase | (i) | Forms phosphodiester bonds |
| 2. | Taq polymerase | (ii) | cDNA library |
| 3. | Alkaline phosphatase | (iii) | Breaks phosphodiester bonds |
| 4. | Restriction endonuclease | (iv) | Used in ELISA |
1. a(ii), b(iii), c(iv), d(i)
2. a(ii), b(i), c(iii), d(iv)
3. a(i), b(ii), c(iii), d(iv)
4. a(ii), b(i), c(iv), d(iii)
Subtopic: Tools |
69%
Please attempt this question first.
Hints
Complete the analogy and select the correct option.
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
Subtopic: Restriction Enzymes - Main Enzymes |
79%
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All of the given statements are true with respect to the separation of DNA fragments by gel electrophoresis except:
| 1. | The cutting of DNA by restriction endonucleases results in fragments of DNA and these fragments can be separated by this technique. |
| 2. | The most commonly used matrix is agarose which is a natural polymer extracted from seaweeds. |
| 3. | The DNA fragments resolve according to their size. Hence, the larger the fragment size, the farther it moves. |
| 4. | The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide. |
Subtopic: Separation and Isolation of DNA fragments |
89%
From NCERT
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Hints
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EcoRI cuts the DNA between bases _______ and ______ only when the recognition sequence GAATTC is present in the DNA.
Select the correct option to fill the blanks respectively.
1. A and T
2. T and C
3. G and A
4. A and C
Select the correct option to fill the blanks respectively.
1. A and T
2. T and C
3. G and A
4. A and C
Subtopic: Tools: Enzymes: I | Restriction Enzymes - Main Enzymes |
78%
From NCERT
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How many cycles of PCR are required for obtaining 256 molecules of dsDNA, if your starting material was one molecule of dsDNA?
1. 4
2. 9
3. 128
4. 8
1. 4
2. 9
3. 128
4. 8
Subtopic: Polymerase Chain Reaction: PCR |
57%
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Hints
Select the incorrect match with respect to rDNA technology:
| 1. | Bioprocess engineering | – | Maintenance of sterile ambience |
| 2. | Genetic engineering | – | Alteration of genetic material |
| 3. | Downstream processing | – | Biosynthetic stage |
| 4. | Biosynthetic stage | – | Culturing of recombinant host cells |
Subtopic: Bioreactors/Downstream Processing |
76%
From NCERT
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