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Q. No.The main challenge for production of insulin using rDNA techniques was:
1. getting insulin assembled into a mature form.
2. isolating DNA in pure form from human cell
3. selecting the vector for cloning the gene
4. purification of the human insulin
Subtopic: Med Applications & Humulin |
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Identify the incorrect statement:
| 1. | PCR is now routinely used to detect HIV in suspected AIDS patients. |
| 2. | PCR is being used to detect mutations in genes in suspected cancer patients. |
| 3. | ELISA is based on the principle of antigen-antibody interaction. |
| 4. | ELISA does not detect antibodies and can detect only antigens. |
Subtopic: Molecular Diagnosis |
89%
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The first transgenic cow produced:
| 1. | enzyme to treat emphysema |
| 2. | human protein enriched milk |
| 3. | enzyme to treat phenylketonuria |
| 4. | organs for xenotransplantation |
Subtopic: Transgenic Microbes & Animals |
93%
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Biopiracy is:
| 1. | the exploration of natural sources for small molecules, macromolecules and biochemical and genetic information that could be developed into commercially valuable products. |
| 2. | exploitation of a region's biological resources or indigenous knowledge unethically and without providing fair compensation. |
| 3. | the use of living organisms, like microbes and bacteria, in the removal of contaminants, pollutants, and toxins from soil, water, and other environments |
| 4. | the process of developing a crop with bioavailable micronutrients in its edible parts. |
Subtopic: Ethical Issues |
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Consider the given two statements:
I. The first clinical gene therapy was given to a girl with adenosine deaminase deficiency
II. The first recombinant therapeutic product approved for human use was Human Insulin
I. The first clinical gene therapy was given to a girl with adenosine deaminase deficiency
II. The first recombinant therapeutic product approved for human use was Human Insulin
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
Subtopic: Gene Therapy |
89%
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India successfully protected itself against biopiracy in a case where in late 1997, the United States Patent and Trademark Office granted a patent to:
1. Co-667
2. Sharbati Sonora
3. Lerma Rojo
4. Basmati
1. Co-667
2. Sharbati Sonora
3. Lerma Rojo
4. Basmati
Subtopic: Ethical Issues |
95%
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ELISA works on the principle of:
| 1. | antigen-antibody interaction |
| 2. | radioactive probe tagging |
| 3. | culture of microbe in an artificial medium |
| 4. | histopathology |
Subtopic: Molecular Diagnosis |
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Identify the incorrect statement regarding Bacillus thuringiensis?
| 1. | forms protein crystals during a particular phase of their growth which contains a toxic insecticidal protein. |
| 2. | The Bt toxin protein exists as an inactive protoxin that is converted into an active form by an alkaline pH in the insect's gut. |
| 3. | Activated toxin binds to the surface of midgut epithelial cells, creates pores, causes cell swelling and lysis and eventually death of the insect. |
| 4. | Proteins encoded by the genes cryIAc and cryIIAb control the corn borer, that of cryIAb controls cotton bollworms. |
Subtopic: Bt Crops |
83%
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RNA interference:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). |
| II: | has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Interference RNA Tech |
92%
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To produce human recombinant insulin, Eli Lily:
| 1. | prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. |
| 2. | prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains. |
| 3. | used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA. |
| 4. | used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells. |
Subtopic: Med Applications & Humulin |
92%
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