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Q. No.Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
Column I
(PCR steps)
Column II
(Temperature)
i.
Polymerization
P.
\(40-60 ^\circ C\)
ii.
Denaturation
Q.
\(70-75^ \circ C\)
iii.
Primer annealing
R.
\(90-98 ^ \circ C \)
S.
\(28-37^ \circ C\)
1. i-S, ii-R, iii-Q
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
(PCR steps)
(Temperature)
Subtopic: Polymerase Chain Reaction: PCR |
64%
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From the following identify the INCORRECT pair.
| Restriction enzyme | Source | |
| 1. | AluI | Arthrobacter luteus |
| 2. | EcoRI | Esherichia coli Ry13 |
| 3. | BamHI | Bacillus thuringiensis H |
| 4. | HindII | H. influenza Rd |
Subtopic: Tools: Vectors: I |
59%
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From the following identify the INCORRECT pair.
| Restriction enzyme | Type of ends produced | |
| 1. | AluI | Blunt ends |
| 2. | EcoRI | Sticky ends |
| 3. | BamHI | Sticky ends |
| 4. | HindII | Sticky ends |
Subtopic: Restriction Enzymes - Main Enzymes |
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Match the following enzymes with their functions:
Select the correct option from the following:
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
| Column I | Column II | ||
| i. | Restriction endonuclease | a. | Joins the DNA fragments |
| ii. | Restriction exonucleases | b. | Extends primers on genomic DNA template |
| iii. | DNA ligase | c. | Cuts DNA at Specific position |
| iv. | Taq polymerase | d. | Removes nucleotides from the ends of DNA |
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
82%
From NCERT
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Study the following lists:
1. (i-c); (ii-e); (iii-d); (iv-b)
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
| List I | List II | ||
| i. | Vector | a. | Resistant to cotton bollworms. |
| ii. | Downstream processing | b. | Mobile genetic elements |
| iii. | Cry II AB | c. | Controls corn borer |
| iv. | Transposons | d. | Ti plasmid |
| e. | Purifying protein in biopharmaceuticals. |
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
Subtopic: Bt Crops |
82%
From NCERT
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Match the following.
The CORRECT answer is
| List I | List II | List II | |||
| i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
| i | ii | iii | iv | |
| 1. | d,1 | a,3 | c,3 | b,4 |
| 2. | d,1 | c,2 | a,3 | b,4 |
| 3. | d,1 | c,2 | b,4 | a,3 |
| 4. | a,3 | b,4 | c,2 | d,1 |
Subtopic: Tools: Vectors: I |
74%
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Study the following lists
| List I | List II | ||
| 1 | Eco RI | I | Vector for transgenic plants |
| 2 | Ti plasmid | II | Restriction enzyme |
| 3 | pBR 322 | III | Probe |
| 4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
| V | Artificial plasmid |
| 1 | 2 | 3 | 4 | |
| 1. | II | I | V | IV |
| 2. | I | II | III | IV |
| 3. | II | I | IV | V |
| 4. | II | I | IV | III |
Subtopic: Tools: Vectors: I |
71%
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Match the technique in columns-I with the terms given in column-II.
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column I | Column II | ||
| (a) | Gel electrophoresis | (i) | Chimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments. |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Tools |
75%
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Match the restriction enzyme given in Column-I with their source given in Column-II and choose the correct option.
1. i-a, ii-c, iii-d, iv-b
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
| Column-I | Column-II | ||
| i. | Alu I | a. | Bacillus amyloliquefaciens H |
| ii. | Bam HI | b. | H. influenza RD |
| iii. | Eco RI | c. | Arthobacter luteus |
| iv. | Hind II | d. | Escherichia coli Ry 13 |
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
77%
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Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and Select the correct option.
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
| Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
| i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
| ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
| iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
| iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
Subtopic: Tools: Vectors: I |
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