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Q. No.Match the following enzymes with their functions:
Column I
Column II
i.
Restriction endonuclease
a.
Joins the DNA fragments
ii.
Restriction exonucleases
b.
Extends primers on genomic DNA template
iii.
DNA ligase
c.
Cuts DNA at Specific position
iv.
Taq polymerase
d.
Removes nucleotides from the ends of DNA
Select the correct option from the following:
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
82%
From NCERT
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Study the following lists:
1. (i-c); (ii-e); (iii-d); (iv-b)
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
| List I | List II | ||
| i. | Vector | a. | Resistant to cotton bollworms. |
| ii. | Downstream processing | b. | Mobile genetic elements |
| iii. | Cry II AB | c. | Controls corn borer |
| iv. | Transposons | d. | Ti plasmid |
| e. | Purifying protein in biopharmaceuticals. |
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
Subtopic: Bt Crops |
82%
From NCERT
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Match the following.
The CORRECT answer is
| List I | List II | List II | |||
| i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
| i | ii | iii | iv | |
| 1. | d,1 | a,3 | c,3 | b,4 |
| 2. | d,1 | c,2 | a,3 | b,4 |
| 3. | d,1 | c,2 | b,4 | a,3 |
| 4. | a,3 | b,4 | c,2 | d,1 |
Subtopic: Tools: Vectors: I |
74%
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Study the following lists
| List I | List II | ||
| 1 | Eco RI | I | Vector for transgenic plants |
| 2 | Ti plasmid | II | Restriction enzyme |
| 3 | pBR 322 | III | Probe |
| 4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
| V | Artificial plasmid |
| 1 | 2 | 3 | 4 | |
| 1. | II | I | V | IV |
| 2. | I | II | III | IV |
| 3. | II | I | IV | V |
| 4. | II | I | IV | III |
Subtopic: Tools: Vectors: I |
71%
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Match the technique in columns-I with the terms given in column-II.
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column I | Column II | ||
| (a) | Gel electrophoresis | (i) | Chimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments. |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Tools |
75%
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Match the restriction enzyme given in Column-I with their source given in Column-II and choose the correct option.
1. i-a, ii-c, iii-d, iv-b
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
| Column-I | Column-II | ||
| i. | Alu I | a. | Bacillus amyloliquefaciens H |
| ii. | Bam HI | b. | H. influenza RD |
| iii. | Eco RI | c. | Arthobacter luteus |
| iv. | Hind II | d. | Escherichia coli Ry 13 |
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
77%
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Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and Select the correct option.
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
| Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
| i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
| ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
| iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
| iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
Subtopic: Tools: Vectors: I |
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Match Column-I with column II and select the correct option
1. (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
| (b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
| (c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
| (d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Tools |
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Match Column-I with column II and select the correct option
1. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophosis | (i) | To combine genes from two organisms |
| (b) | cDNA | (ii) | To make a copy of DNA from mRNA |
| (c) | Genetic Engineering | (iii) | Separate the DNA fragments |
| (d) | PCR | (iv) | Gene multiplication in vitro |
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
Subtopic: Tools |
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Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | 70-75°C |
| (b) | Annealing | (ii) | 90-98°C |
| (c) | Primer extension | (iii) | 40-60°C |
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
66%
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