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Q. No.Assertion(A): Synthetic oligonucleotide polymers are used during Annealing in a PCR
Reason(R): The primers bind to the double stranded DNA at their complementary regions
1.
Both (A) and (R) are true and (R) is the correct explanation of (A)
2.
Both (A) and (R) are true, but (R) is not the correct explanation of (A)
3.
(A) is true, but (R) is false
4.
(A) is false, but (R) is true
Subtopic: Polymerase Chain Reaction: PCR |
From NCERT
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Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
1. i-S, ii-R, iii-Q
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
| Column I (PCR steps) |
Column II (Temperature) |
||
| i. | Polymerization | P. | \(40-60 ^\circ C\) |
| ii. | Denaturation | Q. | \(70-75^ \circ C\) |
| iii. | Primer annealing | R. | \(90-98 ^ \circ C \) |
| S. | \(28-37^ \circ C\) |
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
Subtopic: Polymerase Chain Reaction: PCR |
64%
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From the following identify the INCORRECT pair.
| Restriction enzyme | Source | |
| 1. | AluI | Arthrobacter luteus |
| 2. | EcoRI | Esherichia coli Ry13 |
| 3. | BamHI | Bacillus thuringiensis H |
| 4. | HindII | H. influenza Rd |
Subtopic: Tools: Vectors: I |
59%
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From the following identify the INCORRECT pair.
| Restriction enzyme | Type of ends produced | |
| 1. | AluI | Blunt ends |
| 2. | EcoRI | Sticky ends |
| 3. | BamHI | Sticky ends |
| 4. | HindII | Sticky ends |
Subtopic: Restriction Enzymes - Main Enzymes |
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Match the following enzymes with their functions:
Select the correct option from the following:
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
| Column I | Column II | ||
| i. | Restriction endonuclease | a. | Joins the DNA fragments |
| ii. | Restriction exonucleases | b. | Extends primers on genomic DNA template |
| iii. | DNA ligase | c. | Cuts DNA at Specific position |
| iv. | Taq polymerase | d. | Removes nucleotides from the ends of DNA |
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
82%
From NCERT
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Study the following lists:
1. (i-c); (ii-e); (iii-d); (iv-b)
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
| List I | List II | ||
| i. | Vector | a. | Resistant to cotton bollworms. |
| ii. | Downstream processing | b. | Mobile genetic elements |
| iii. | Cry II AB | c. | Controls corn borer |
| iv. | Transposons | d. | Ti plasmid |
| e. | Purifying protein in biopharmaceuticals. |
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
Subtopic: Bt Crops |
82%
From NCERT
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Match the following.
The CORRECT answer is
| List I | List II | List II | |||
| i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
| i | ii | iii | iv | |
| 1. | d,1 | a,3 | c,3 | b,4 |
| 2. | d,1 | c,2 | a,3 | b,4 |
| 3. | d,1 | c,2 | b,4 | a,3 |
| 4. | a,3 | b,4 | c,2 | d,1 |
Subtopic: Tools: Vectors: I |
74%
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Study the following lists
| List I | List II | ||
| 1 | Eco RI | I | Vector for transgenic plants |
| 2 | Ti plasmid | II | Restriction enzyme |
| 3 | pBR 322 | III | Probe |
| 4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
| V | Artificial plasmid |
| 1 | 2 | 3 | 4 | |
| 1. | II | I | V | IV |
| 2. | I | II | III | IV |
| 3. | II | I | IV | V |
| 4. | II | I | IV | III |
Subtopic: Tools: Vectors: I |
71%
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Match the technique in columns-I with the terms given in column-II.
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column I | Column II | ||
| (a) | Gel electrophoresis | (i) | Chimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments. |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Tools |
75%
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Match the restriction enzyme given in Column-I with their source given in Column-II and choose the correct option.
1. i-a, ii-c, iii-d, iv-b
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
| Column-I | Column-II | ||
| i. | Alu I | a. | Bacillus amyloliquefaciens H |
| ii. | Bam HI | b. | H. influenza RD |
| iii. | Eco RI | c. | Arthobacter luteus |
| iv. | Hind II | d. | Escherichia coli Ry 13 |
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
77%
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